ABSTRACT
The purpose of the present study was to detected humoral immunity based on antibody deposit of chickens egg yolk exposed to excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of 6 head chickens were devided into two groups. First group, the chickens were not immnunized. Second group, the chickens were immunized with excretory/secretory. Active immunizations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by Agar Gel Precipitation Test (AGPT). The result showed that antibody in yolk was begun detect with AGPT at four weeks, and antibody recognized by excretory/secretory until five and six weeks after immunization, This study has shown that the excretory/secretory released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.
Key words: Ascaridia galli, excretory/secretory, antibody, yolk
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